HOW TO PLOT MELT CURVE CFX MANAGER PDF

Results: The assay had a reproducibility of 100%, could detect gDNA of different genotype as low as 1 ng per reaction, and had an overall accuracy of 100% when compared with RDB analysis and Sanger sequencing. The sensitivity, reproducibility, and accuracy of the method were validated via 700 genomic DNA samples. Using the HRM software, the aligned melt curve and the difference plot of each sample were compared with the negative and positive control. CFX Manager software is run on a personal computer (PC) with either the Windows XP, Windows 7, or Windows 8 operating system and is required to analyze real-time PCR data from the CFX96 Touch system, CFX96 Touch Deep Well system, CFX Connect system, or CFX384 Touch system. Methods: The assay consisted of one pair of primers specific for the HBA 2 gene and four dual-labeled, self-quenched probes targeting six non-deletional О± -thalassemia mutations. The result of HRMA and Tm-shift qPCR were analyzed using the high resolution melt software v3.1 and the Bio-Rad CFX Manager software, respectively. In this study, a closed- and single-tube assay for the detection of six common non-deletional О± -thalassemia mutations in the HBA 2 gene was developed based on multicolor melting curve analysis. To explain these apparently conflicting results, we need to examine how the melt curves are produced. However, non-deletional О± -thalassemia mutations remain undetected in routine clinical testing due to the lack of a suitable method. Below is a sample Custom Data View of a gene expression experiment, including an Amplification.

HOW TO PLOT MELT CURVE CFX MANAGER PDF

pdf file), create and copy graphs, get information from melt curves and standard curves, view quantification data, etc. Huang, Qiuying Wang, Xudong Tang, Ning Zhu, Chunjiang Yan, Tizhen Chen, Ping Li, QinggeДЄbstract Background: О± -Thalassemia, caused by mutations in the О±-globin genes, is one of the most common monogenic inherited disorders in the world. The Data Analysis window can be used to view and analyze your data, create output reports (click the Reports button to save as a. Rapid detection of non-deletional mutations causing О± -thalassemia by multicolor melting curve analysis Rapid detection of non-deletional mutations causing О± -thalassemia by multicolor melting curve.

How to plot melt curve cfx manager